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KMID : 0369319920120030270
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Allergy
1992 Volume.12 No. 3 p.270 ~ p.284
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Platelet Activating Factor(PAF) in An Eosinophil Activator in Asthma
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Abstract
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In asthma, PAF is one of potent bronchoconstrictro, and it induces non-specific bronchial hyperresponsiveness which is the fundamental charcteristic pathophysiology of asthma. PAF is poten chemoattarctant of eosinophil which is the main
inflammatory
effector cell in rate asthmatic creaction However not only the activation process of imchanism of non=specific bronchial hyperresmpnsiveness have not been clarified ver. To investigate the role of PAF on eosinophil activation, eosinophils
obtained
from
peripheral blood of the patients with asthma were purified by discontinous percoll gradient (91±4%), and PAF-induced eosinophil chemotaxis, LTC4 generation and ECP release from eosinophil were measured by radioimmunoassay.
@ES The results were as follows:
@EN 1) PAF-induced eosinophil chemotaxis appeared at 10E-10 M, and this effect was increased up to 10E-6M in dose-response manner.
2) PAF-induced LTC4 production began at 10E-8M and that was increased up to 10E-5M in dose-response manner.
3) PAF-induced ECP release also began at 10E-8M and increased up to 10E-5M in does-response manner.
Taken together PAF induces migration of eosinophil into the bronchus and PAF activates eosinophils through PAF-receptor binding on eosinophils, and non-specific bronchial hyperresponsiveness developes through this eosinophil-induced airway
inflammation.±±
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KEYWORD
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